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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 26652 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCFP-N1
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameβ3-integrin
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SpeciesH. sapiens (human)
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Entrez GeneITGB3 (a.k.a. BDPLT16, BDPLT2, BDPLT24, CD61, GP3A, GPIIIa, GT, GT2)
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Tag
/ Fusion Protein
- CFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site BglII (destroyed during cloning)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer EGFP-N (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
β3-integrin-CFP was a gift from Jonathan Jones (Addgene plasmid # 26652 ; http://n2t.net/addgene:26652 ; RRID:Addgene_26652) -
For your References section:
Microfilament-dependent movement of the beta3 integrin subunit within focal contacts of endothelial cells. Tsuruta D, Gonzales M, Hopkinson SB, Otey C, Khuon S, Goldman RD, Jones JC. FASEB J. 2002 Jun . 16(8):866-8. 10.1096/fj.01-0878fje PubMed 11967230