pRL-TK 3'FBXW7 UTR
(Plasmid #26649)

• Depositing Lab: Alex Minella
• Publication: Xu et al J Biol Chem. 2010 Sep 7. ():.

Backbone

• Vector backbone: pRL-TK
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4045
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FBXW7
• Species: H. sapiens (human)
• Insert Size (bp): 1571
• Mutation: Contains only the 3'UTR of FBXW7, nucleotides 1-1571 following stop codon.
• Entrez Gene: FBXW7 (a.k.a. AGO, CDC4, DEDHIL, FBW6, FBW7, FBX30, FBXO30, FBXW6, SEL-10, SEL10, hAgo, hCdc4)
• Tag / Fusion Protein:
  • Luciferase (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: ApaI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: EBV-Rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene's sequencing results identified nucleotide mismatches when compared to GenBank reference sequence NM_018315.4 at basepair #'s 2389, 2663, 2666, 2829 and 3526.