pCAG-Cre-IRES2-GFP
(Plasmid #26646)

• Depositing Lab: Anjen Chenn
• Publication: Woodhead et al J Neurosci. 2006 Nov 29;26(48):12620-30.

Backbone

• Vector backbone: pCAG-IRES2-GFP
• Backbone size w/o insert (bp): 6000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cre
• Alt name: Cre recombinase
• Species: Bacteriophage P1
• Insert Size (bp): 1000
• Tags / Fusion Proteins:
  • IRES2 (C terminal on insert)
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: pCAG-Fwd
• 3′ sequencing primer: GFP-R

Resource Information

• Articles Citing this Plasmid:
  • 25 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Expresses Cre and GFP (driven by IRES)