pZE21-GFPaav
(Plasmid #26643)

• Depositing Lab: Michael Elowitz
• Publication: Elowitz et al Nature. 2000 Jan 20. 403(6767):335-8.

Backbone

• Vector backbone: pZE21
• Backbone size w/o insert (bp): 2117
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: pZE21-GFPaav
• Alt name: reporter
• Species: e. coli
• Insert Size (bp): 865
• Mutation: na
• Tag / Fusion Protein:
  • GFPaav

Cloning Information

• Cloning method: Restriction Enzyme
• 3′ cloning site: hindIII (not destroyed)
• 5′ sequencing primer: GFP-F

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note that there are some discrepancies between Addgene's QC sequence and the depositor's sequence. These differences are not known to affect function.