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Addgene

pET21b Apaf1 1-591
(Plasmid #26642)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26642 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5443
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Apoptotic protease activating factor 1
  • Alt name
    Apaf1
  • Alt name
    CED4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1800
  • Mutation
    Amino Acids 1-591
  • Entrez Gene
    APAF1 (a.k.a. APAF-1, CED4)
  • Tag / Fusion Protein
    • His (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7 forward
  • 3′ sequencing primer T7 reverse
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET21b Apaf1 1-591 was a gift from Guy Salvesen (Addgene plasmid # 26642 ; http://n2t.net/addgene:26642 ; RRID:Addgene_26642)

  • For your References section:

    The apoptosome activates caspase-9 by dimerization. Pop C, Timmer J, Sperandio S, Salvesen GS. Mol Cell. 2006 Apr 21. 22(2):269-75. 10.1016/j.molcel.2006.03.009 PubMed 16630894
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