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pLJM1 p18 mito
(Plasmid #26638)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26638 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLJM1
  • Backbone manufacturer
    Available at Addgene (#19319)
  • Backbone size w/o insert (bp) 7300
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Grow in XL10Gold or Stbl3 cells to decrease recombination. Growing in SOC also helps.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    p18 mito
  • Alt name
    p18
  • Alt name
    c11orf59
  • Species
    H. sapiens (human)
  • Entrez Gene
    LAMTOR1 (a.k.a. C11orf59, PDRO, Ragulator1, p18, p27RF-Rho)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The p18 mito expression
plasmid was generated by cloning of a mutant p18 with amino acids 2–4 changed to alanines into a modified version of the pLKO.1 vector that added, to the C terminus of p18, the mitochondrial localization signal of OMP25
protein.

The order of the construct is the following: Age1-flag-sal-p18(residues 2-4 mutated to alanine, stop condon removed)-GGGGS (2X polylinker)-omp25 seq-stop codon-ecor1

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLJM1 p18 mito was a gift from David Sabatini (Addgene plasmid # 26638 ; http://n2t.net/addgene:26638 ; RRID:Addgene_26638)

  • For your References section:

    Ragulator-Rag complex targets mTORC1 to the lysosomal surface and is necessary for its activation by amino acids. Sancak Y, Bar-Peled L, Zoncu R, Markhard AL, Nada S, Sabatini DM. Cell. 2010 Apr 16. 141(2):290-303. 10.1016/j.cell.2010.02.024 PubMed 20381137
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