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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 26637 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLJM1
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Backbone manufacturerAvailable at Addgene (#19319)
- Backbone size w/o insert (bp) 7400
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Growth instructionsGrow in XL10Gold or Stbl3 cells to decrease recombination. Growing the cultures at 30C rather than 37 and in SOC sometimes helps with yield.
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameraptor-HRas25
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Alt nameraptor HRas25
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SpeciesH. sapiens (human)
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Entrez GeneRPTOR (a.k.a. KOG1, Mip1)
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Tag
/ Fusion Protein
- Flag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (not destroyed)
- 3′ cloning site BstBI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The insert is structured:
AgeI-FlagTag-SalI-Raptor without stop codon-EcoRV-localization tag-stop codon-BstBI.
New genes can be cloned into the vector with N-term Flag and C-term localization
signal using SalI-EcoRV.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLJM1 Flag Raptor HRas25 was a gift from David Sabatini (Addgene plasmid # 26637 ; http://n2t.net/addgene:26637 ; RRID:Addgene_26637) -
For your References section:
Ragulator-Rag complex targets mTORC1 to the lysosomal surface and is necessary for its activation by amino acids. Sancak Y, Bar-Peled L, Zoncu R, Markhard AL, Nada S, Sabatini DM. Cell. 2010 Apr 16. 141(2):290-303. 10.1016/j.cell.2010.02.024 PubMed 20381137
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/98/08/a215c268-af63-11e0-90fe-003048dd6500.jpeg.940x940_q85_autocrop.png)