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Addgene

pLJM1 Flag Raptor HRas25
(Plasmid #26637)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26637 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLJM1
  • Backbone manufacturer
    Available at Addgene (#19319)
  • Backbone size w/o insert (bp) 7400
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Grow in XL10Gold or Stbl3 cells to decrease recombination. Growing the cultures at 30C rather than 37 and in SOC sometimes helps with yield.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    raptor-HRas25
  • Alt name
    raptor HRas25
  • Species
    H. sapiens (human)
  • Entrez Gene
    RPTOR (a.k.a. KOG1, Mip1)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site BstBI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The insert is structured:

AgeI-FlagTag-SalI-Raptor without stop codon-EcoRV-localization tag-stop codon-BstBI.

New genes can be cloned into the vector with N-term Flag and C-term localization
signal using SalI-EcoRV.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLJM1 Flag Raptor HRas25 was a gift from David Sabatini (Addgene plasmid # 26637 ; http://n2t.net/addgene:26637 ; RRID:Addgene_26637)

  • For your References section:

    Ragulator-Rag complex targets mTORC1 to the lysosomal surface and is necessary for its activation by amino acids. Sancak Y, Bar-Peled L, Zoncu R, Markhard AL, Nada S, Sabatini DM. Cell. 2010 Apr 16. 141(2):290-303. 10.1016/j.cell.2010.02.024 PubMed 20381137
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