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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 26616 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepKSV7
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)HB101
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Growth instructionsIn HB101 cells. Use 50ug/ml Amp
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Copy numberUnknown
Gene/Insert
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Gene/Insert namegfpuv
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Tag
/ Fusion Protein
- gram-positive ribosome binding site (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site PstI (not destroyed)
- 5′ sequencing primer GFP-F (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bygfpuv coding sequence was amplified from plasmid pGFPuv (Clontech).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Gram-positive ribosome binding site (AGGAGGA) derived from SD1 of ermC.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNF579 was a gift from Nancy Freitag (Addgene plasmid # 26616 ; http://n2t.net/addgene:26616 ; RRID:Addgene_26616) -
For your References section:
Listeria monocytogenes-infected phagocytes can initiate central nervous system infection in mice. Drevets DA, Jelinek TA, Freitag NE. Infect Immun. 2001 Mar . 69(3):1344-50. 10.1128/IAI.69.3.1344-1350.2001 PubMed 11179297