pcDNA3-Flag mTOR wt Notes
Addgene Diagnostic Digest for 26603
This gel depicts the NEB 1kb ladder and a digestion of the plasmid 26603 with the NotI-HF enzyme. The expected pattern of bands was observed.
Addgene Diagnostic Digest
This gel depicts the NEB 1kb DNA ladder and a digestion of plasmid 26603 with EcoRI-HF.
Expected banding pattern:
EcoRI-HF: 3 bands at 9, 3 and 1 kb.
Addgene Diagnostic Digest
This gel depicts the NEB 1kb ladder and a digestion of the plasmid pcDNA3-Flag mTOR wt with the following enzymes: HpaI and PvuI-HF.