pcDNA3-Flag mTOR wt
(Plasmid #26603)

• Purpose: Mammalian expression of human flag-tagged mTor
• Depositing Lab: Jie Chen
• Publication: Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: mTOR
• Species: H. sapiens (human)
• Insert Size (bp): 7650
• GenBank ID: NM_004958
• Entrez Gene: MTOR (a.k.a. FRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS)
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest for 26603
  • Addgene Diagnostic Digest
  • Addgene Diagnostic Digest
• A portion of this plasmid was derived from a plasmid made by: WT mTOR insert is from Stuart Schreiber's lab. Brown et al, Nature 369:756 (1994).
• Articles Citing this Plasmid:
  • 31 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry