nanos-PCRII
(Plasmid
#26601)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 26601 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonePCRII
- Backbone size w/o insert (bp) 4000
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namenanos
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SpeciesS. mediterranea (planarian)
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Insert Size (bp)918
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GenBank IDef035555
Cloning Information
- Cloning method TOPO Cloning
- 5′ cloning site T/A Cloning (destroyed during cloning)
- 3′ cloning site T/A Cloning (destroyed during cloning)
- 5′ sequencing primer SP6
- 3′ sequencing primer M13pUC-fwd (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
nanos-PCRII was a gift from Phillip Newmark (Addgene plasmid # 26601 ; http://n2t.net/addgene:26601 ; RRID:Addgene_26601) -
For your References section:
nanos function is essential for development and regeneration of planarian germ cells. Wang Y, Zayas RM, Guo T, Newmark PA. Proc Natl Acad Sci U S A. 2007 Apr 3. 104(14):5901-6. 10.1073/pnas.0609708104 PubMed 17376870