pBS-germinal histone H4
(Plasmid
#26600)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 26600 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBluescript SKII (+)
- Backbone size w/o insert (bp) 3000
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namegerminal histone H4
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SpeciesS. mediterranea (planarian)
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Insert Size (bp)926
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GenBank IDDN306099
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Addgene's quality control sequence has minor discrepancies with the provided depositor's sequence; these do not affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS-germinal histone H4 was a gift from Phillip Newmark (Addgene plasmid # 26600 ; http://n2t.net/addgene:26600 ; RRID:Addgene_26600) -
For your References section:
nanos function is essential for development and regeneration of planarian germ cells. Wang Y, Zayas RM, Guo T, Newmark PA. Proc Natl Acad Sci U S A. 2007 Apr 3. 104(14):5901-6. 10.1073/pnas.0609708104 PubMed 17376870