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Addgene

pBS-germinal histone H4
(Plasmid #26600)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26600 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBluescript SKII (+)
  • Backbone size w/o insert (bp) 3000

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    germinal histone H4
  • Species
    S. mediterranea (planarian)
  • Insert Size (bp)
    926
  • GenBank ID
    DN306099

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene's quality control sequence has minor discrepancies with the provided depositor's sequence; these do not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS-germinal histone H4 was a gift from Phillip Newmark (Addgene plasmid # 26600 ; http://n2t.net/addgene:26600 ; RRID:Addgene_26600)
  • For your References section:

    nanos function is essential for development and regeneration of planarian germ cells. Wang Y, Zayas RM, Guo T, Newmark PA. Proc Natl Acad Sci U S A. 2007 Apr 3. 104(14):5901-6. 10.1073/pnas.0609708104 PubMed 17376870