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Addgene

pCAGTetRnls
(Plasmid #26599)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26599 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAG
  • Backbone size w/o insert (bp) 6414
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Tetracycline repressor
  • Alt name
    TetR
  • Species
    E.Coli
  • Insert Size (bp)
    704
  • GenBank ID
    X00694
  • Entrez Gene
    TetR (a.k.a. IPF_209)
  • Tag / Fusion Protein
    • nls (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAGTetRnls was a gift from Peter Andrews (Addgene plasmid # 26599 ; http://n2t.net/addgene:26599 ; RRID:Addgene_26599)

  • For your References section:

    Specific knockdown of OCT4 in human embryonic stem cells by inducible short hairpin RNA interference. Zafarana G, Avery SR, Avery K, Moore HD, Andrews PW. Stem Cells. 2009 Apr . 27(4):776-82. 10.1002/stem.5 PubMed 19350677
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