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Addgene

pZS2-123
(Plasmid #26598)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26598 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pZS2
  • Backbone size w/o insert (bp) 3288
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Synthetic
  • Alt name
    CFP, YFP, RFP
  • Species
    Multiple synthetic genes
  • Insert Size (bp)
    3518
  • Mutation
    N/A

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer see text
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pZS2-123 was a gift from Michael Elowitz (Addgene plasmid # 26598 ; http://n2t.net/addgene:26598 ; RRID:Addgene_26598)
  • For your References section:

    A synthetic three-color scaffold for monitoring genetic regulation and noise. Cox RS, Dunlop MJ, Elowitz MB. J Biol Eng. 2010 . 4():10. 10.1186/1754-1611-4-10 PubMed 20646328