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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 26598 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepZS2
- Backbone size w/o insert (bp) 3288
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameSynthetic
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Alt nameCFP, YFP, RFP
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SpeciesMultiple synthetic genes
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Insert Size (bp)3518
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MutationN/A
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site NheI (not destroyed)
- 5′ sequencing primer see text (Common Sequencing Primers)
Resource Information
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Addgene Notes
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A portion of this plasmid was derived from a plasmid made byDNA2.0 (Menlo Park, CA)
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pZS2-123 was a gift from Michael Elowitz (Addgene plasmid # 26598 ; http://n2t.net/addgene:26598 ; RRID:Addgene_26598) -
For your References section:
A synthetic three-color scaffold for monitoring genetic regulation and noise. Cox RS, Dunlop MJ, Elowitz MB. J Biol Eng. 2010 . 4():10. 10.1186/1754-1611-4-10 PubMed 20646328