Nanog-luciferase targeting construct
(Plasmid
#26591)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 26591 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSP72
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 2394
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Vector typeBacterial Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNanog promoter-luciferase-lox-pgk-neomycin-loc
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SpeciesM. musculus (mouse)
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Insert Size (bp)6331
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site ClaI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Nanog-luciferase targeting construct was a gift from Rudolf Jaenisch (Addgene plasmid # 26591 ; http://n2t.net/addgene:26591 ; RRID:Addgene_26591) -
For your References section:
Reprogramming of murine fibroblasts to induced pluripotent stem cells with chemical complementation of Klf4. Lyssiotis CA, Foreman RK, Staerk J, Garcia M, Mathur D, Markoulaki S, Hanna J, Lairson LL, Charette BD, Bouchez LC, Bollong M, Kunick C, Brinker A, Cho CY, Schultz PG, Jaenisch R. Proc Natl Acad Sci U S A. 2009 Jun 2. 106(22):8912-7. 10.1073/pnas.0903860106 PubMed 19447925
