pSMT3-M
(Plasmid #26589)

• Depositing Lab: Tanya Parish
• Publication: Carroll et al PLoS One. 2010 . 5(3):e9823.

Backbone

• Vector backbone: pSMT3
• Backbone size w/o insert (bp): 5708
• Modifications to backbone: High copy number derivative of pSMT3 constructed by removal of an arginine residue at position 364 in the pAL5000 replicon (A364).
• Vector type: Shuttle vector for mycobacteria
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Hygromycin, 200 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: n/a

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Creation and characterisation of a high-copy-number version of the pAL5000 mycobacterial replicon. Tuberculosis (Edinb) 87: 481–488.

There are known discrepancies between the Addgene QC sequence and the depositor's full sequence--these are likely due to how the full sequence was assembled and should not affect function.