pGEX iLOV
(Plasmid #26587)

• Depositing Lab: John Christie
• Publication: Chapman et al Proc Natl Acad Sci U S A. 2008 Dec 16. 105(50):20038-43.

Backbone

• Vector backbone: pGEX-4T-1
• Backbone manufacturer: GE Healthcare
• Backbone size w/o insert (bp): 4900
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: iLOV
• Alt name: Improved Lov
• Alt name: formerly Phot2
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 330
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: pGEX-5
• 3′ sequencing primer: pGEX-3

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry