pLenti CMVtight eGFP Neo (w784-1)
(Plasmid #26586)

• Purpose: 2nd generation lentiviral vector
• Depositing Lab: Eric Campeau
• Publication: Tet-On Advanced vectors (unpublished)

Backbone

• Vector backbone: p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
• Backbone size w/o insert (bp): 8045
• Vector type: Mammalian Expression, Lentiviral ; Tet-On Advanced vector
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Stbl3, 37oC.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Enhanced Green Fluorescent Protein
• Alt name: eGFP
• Insert Size (bp): 719

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Bst BI (not destroyed)
• 3′ cloning site: Xba I (not destroyed)
• 5′ sequencing primer: CMVtightFORW
• 3′ sequencing primer: WPRErev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The CMVtight element was a gift from Dr. Dominic Esposito.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Need to be transduced in a Tet-On Advanced cell line for expression of GFP.

There are a few mismatches between Addgene's sequence and Dr Campeau's sequence. The sequence differences do not affect GFP expression or general plasmid function.