pPSSUB-101
(Plasmid #26580)

• Depositing Lab: Ron Weiss
• Publication: Basu et al Proc Natl Acad Sci U S A. 2004 Apr 27. 101(17):6355-60.

Backbone

• Vector backbone: pLuxI-tet8 + pRKM-102
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: destabilized GFP
• Alt name: GFP(LVA)
• Species: Aequorea victoria
• Insert Size (bp): 755
• Mutation: S2R, C-terminal extension RPAANDENYA(LVA)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A novel hybrid promoter luxPRcI-OR1 (wild-type luxPR promoter with a CI OR1 operator site inserted at the +1 transcription start) drives expression of GFP(LVA). The promoter was constructed by encoding OR1 on PCR primers and inserting it after luxPR