CaV2.1 (pcDNA6)
(Plasmid #26578)

• Depositing Lab: Diane Lipscombe
• Publication: Richards et al Eur J Neurosci. 2007 Nov . 26(10):2950-61.

Backbone

• Vector backbone: pcDNA6/V5-HisA
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5100
• Vector type: Mammalian Expression
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10F'
• Growth instructions: Top10F'
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Cacna1a
• Alt name: calcium channel, voltage-dependent, P/Q type, alpha 1A subunit
• Species: M. musculus (mouse)
• Insert Size (bp): 7100
• Entrez Gene: Cacna1a (a.k.a. APCA, BI, Caca1a, Cacnl1a4, Cav2.1, Ccha1a, EA2, FHM, HPCA, MHP, MHP1, SCA6, alpha1A, la, nmf352, rkr, smrl, tg)
• Tags / Fusion Proteins:
  • V5 (C terminal on backbone)
  • His6 (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The fragment was amplified from mouse brain. The GenBank sequence is AY714490.