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CaV1.3e[8a,11,31b,Δ32,Δ42a] mut
(Plasmid #26577)

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Item Catalog # Description Quantity Price (USD)
Plasmid 26577 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA6/V5-His ABC
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5100
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10F'
  • Growth instructions
    Top10F'
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CACNA1D
  • Alt name
    calcium channel, voltage-dependent, L type, alpha 1D subunit
  • Alt name
    Cav1.3
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    6400
  • Mutation
    Contains exons 8a, 11, and 31b.Exons 32 and 42a are deleted. Exon 42a was replaced by exons 42 & 49. nt 6224 (GCC->GTC) [Ala->Val]; nt 3310 (GTG->GCG) [Val->Ala]; nt 731(TCA->GGA) [Ser->Gly].
  • Entrez Gene
    Cacna1d

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site SacI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Isolated from superior cervical ganglia.

For more information about this plasmid: http://neuroscience.brown.edu/LipscombeLab/homepage/otherpages/clonespage/clonespage1.htm

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CaV1.3e[8a,11,31b,Δ32,Δ42a] mut was a gift from Diane Lipscombe (Addgene plasmid # 26577 ; http://n2t.net/addgene:26577 ; RRID:Addgene_26577)

  • For your References section:

    Neuronal Ca(V)1.3alpha(1) L-type channels activate at relatively hyperpolarized membrane potentials and are incompletely inhibited by dihydropyridines. Xu W, Lipscombe D. J Neurosci. 2001 Aug 15. 21(16):5944-51. PubMed 11487617
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