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CaV1.3e[8a,11,31b,Δ32,42a] mut
(Plasmid #26576)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26576 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA6/V5-His ABC
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5100
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10F'
  • Growth instructions
    Top10F'
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CACNA1D
  • Alt name
    calcium channel, voltage-dependent, L type, alpha 1D subunit
  • Alt name
    Cav1.3
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    5700
  • Mutation
    Contains exons 8a, 11, 31b and 42a.Deletion of exon 32. nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].
  • Entrez Gene
    Cacna1d
  • Tags / Fusion Proteins
    • V5 (C terminal on backbone)
    • 6XHis (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Isolated from superior cervical ganglia.

For more information about this plasmid: http://neuroscience.brown.edu/LipscombeLab/homepage/otherpages/clonespage/clonespage1.htm

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CaV1.3e[8a,11,31b,Δ32,42a] mut was a gift from Diane Lipscombe (Addgene plasmid # 26576 ; http://n2t.net/addgene:26576 ; RRID:Addgene_26576)

  • For your References section:

    Neuronal Ca(V)1.3alpha(1) L-type channels activate at relatively hyperpolarized membrane potentials and are incompletely inhibited by dihydropyridines. Xu W, Lipscombe D. J Neurosci. 2001 Aug 15. 21(16):5944-51. PubMed 11487617
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