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Addgene

CaVα2δ1
(Plasmid #26575)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26575 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1/Hygro
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5600
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10F'
  • Growth instructions
    TOP10F'
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cacna2d1
  • Alt name
    voltage-gated calcium channel alpha2/delta-1 subunit
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    3965
  • Entrez Gene
    Cacna2d1 (a.k.a. CCHLA2, Cacna2, DHSCCA)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (unknown if destroyed)
  • 3′ cloning site ApaI (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

GENBANK #AF286488

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CaVα2δ1 was a gift from Diane Lipscombe (Addgene plasmid # 26575 ; http://n2t.net/addgene:26575 ; RRID:Addgene_26575)

  • For your References section:

    Alternative splicing in the voltage-sensing region of N-Type CaV2.2 channels modulates channel kinetics. Lin Y, McDonough SI, Lipscombe D. J Neurophysiol. 2004 Nov . 92(5):2820-30. 10.1152/jn.00048.2004 PubMed 15201306
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