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Addgene

CaV1.2
(Plasmid #26572)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26572 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA6/V5-His
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5100
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10F'
  • Growth instructions
    Top10F'
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Calcium channel, voltage-dependent, L type, alpha 1C subunit
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    6600
  • Entrez Gene
    Cacna1c (a.k.a. Cav1.2, Cchl1a1, D930026N18Rik, MBC, MELC-CC)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

GenBank Accesssion number: AY728090

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CaV1.2 was a gift from Diane Lipscombe (Addgene plasmid # 26572 ; http://n2t.net/addgene:26572 ; RRID:Addgene_26572)

  • For your References section:

    Neuronal L-type calcium channels open quickly and are inhibited slowly. Helton TD, Xu W, Lipscombe D. J Neurosci. 2005 Nov 2. 25(44):10247-51. 10.1523/JNEUROSCI.1089-05.2005 PubMed 16267232

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