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Cav2.2e[Δa10, Δ18a, 24a, Δ31a, 37b, 46]
(Plasmid #26571)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26571 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA6/V5-His ABC
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5100
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10F'
  • Growth instructions
    Top10F'
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    calcium channel alpha-1B subunit
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    7390
  • Mutation
    Deletion of exons a10, 18a, and 31a Contains exons 24a, 37b and 46.
  • GenBank ID
    AF055477
  • Entrez Gene
    Cacna1b (a.k.a. BIII)
  • Tags / Fusion Proteins
    • V5 (C terminal on backbone)
    • 6XHis (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site EcorV (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Isolated from superior cervical ganglia.

For more information about this plasmid: http://neuroscience.brown.edu/LipscombeLab/homepage/otherpages/clonespage/clonespage1.htm

Also see: GENBANK AF222337

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Cav2.2e[Δa10, Δ18a, 24a, Δ31a, 37b, 46] was a gift from Diane Lipscombe (Addgene plasmid # 26571 ; http://n2t.net/addgene:26571 ; RRID:Addgene_26571)

  • For your References section:

    Identification of functionally distinct isoforms of the N-type Ca2+ channel in rat sympathetic ganglia and brain. Lin Z, Haus S, Edgerton J, Lipscombe D. Neuron. 1997 Jan . 18(1):153-66. 10.1016/S0896-6273(01)80054-4 PubMed 9010213
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