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Cav2.2e[a10, 18a, Δ24a, 31a, 37b, 46]
(Plasmid #26568)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26568 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA6/V5-His ABC
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5100
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10F'
  • Growth instructions
    Top10F'
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    calcium channel alpha-1B subunit
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    7390
  • Mutation
    contains exons a10, 18a, 31a, 37b, and 46. Deletion of exon 24a
  • GenBank ID
    AF055477
  • Entrez Gene
    Cacna1b (a.k.a. BIII)
  • Tags / Fusion Proteins
    • V5 (C terminal on backbone)
    • 6XHis (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site EcorV (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Isolated from superior cervical ganglia.

For more information about this plasmid: http://neuroscience.brown.edu/LipscombeLab/homepage/otherpages/clonespage/clonespage1.htm

Also see: GENBANK AF222337

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Cav2.2e[a10, 18a, Δ24a, 31a, 37b, 46] was a gift from Diane Lipscombe (Addgene plasmid # 26568 ; http://n2t.net/addgene:26568 ; RRID:Addgene_26568)

  • For your References section:

    Alternative splicing in the cytoplasmic II-III loop of the N-type Ca channel alpha 1B subunit: functional differences are beta subunit-specific. Pan JQ, Lipscombe D. J Neurosci. 2000 Jul 1. 20(13):4769-75. PubMed 10864934
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