PP1 7-300
(Plasmid #26566)

• Depositing Lab: Wolfgang Peti
• Publication: Kelker et al J Mol Biol. 2009 Jan 9. 385(1):11-21.

Backbone

• Vector backbone: RP1B
• Backbone manufacturer: Peti Lab (Addgene #26563)
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PP1a
• Alt name: PP1 alpha
• Species: H. sapiens (human)
• Insert Size (bp): 879
• Mutation: Contains only aa 7-330
• Entrez Gene: PPP1CA (a.k.a. PP-1A, PP1A, PP1alpha, PPP1A)
• Tags / Fusion Proteins:
  • Thio-6 (N terminal on backbone)
  • His6 (N terminal on backbone)
  • TEV cleavage site (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The backbone is a modified version of pET28 and is also available at Addgene (plasmid #26563)