RP1B
(Plasmid
#26563)
-
Purpose(Empty Backbone)
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 26563 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepET28
-
Backbone manufacturerStratagene
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameNone
-
Alt namepETRP1
-
Tags
/ Fusion Proteins
- Thio-6 (N terminal on backbone)
- His6 (N terminal on backbone)
- TEV cleavage site (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid is referred to as pETRP1 in the publication.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
RP1B was a gift from Wolfgang Peti (Addgene plasmid # 26563 ; http://n2t.net/addgene:26563 ; RRID:Addgene_26563) -
For your References section:
Strategies to maximize heterologous protein expression in Escherichia coli with minimal cost. Peti W, Page R. Protein Expr Purif. 2007 Jan . 51(1):1-10. 10.1016/j.pep.2006.06.024 PubMed 16904906