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pDP-let-7_wt
(Plasmid #26558)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26558 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDP19
  • Backbone manufacturer
    Ambion
  • Backbone size w/o insert (bp) 2700
  • Vector type
    transcription vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    cel-pre-let-7
  • Alt name
    pre let-7
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    71

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer M13 reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This construct does not produce perfect pre-let-7. There are leader and tail sequences from the backbone MCS.

Loop mutants have also all been cloned into this backbone for in vitro translation.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDP-let-7_wt was a gift from Chang-Zheng Chen (Addgene plasmid # 26558 ; http://n2t.net/addgene:26558 ; RRID:Addgene_26558)

  • For your References section:

    The potential functions of primary microRNAs in target recognition and repression. Trujillo RD, Yue SB, Tang Y, O'Gorman WE, Chen CZ. EMBO J. 2010 Aug 31. ():. 10.1038/emboj.2010.208 PubMed 20808284
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