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Addgene

pT2/SVNeo
(Plasmid #26553)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26553 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3712
  • Vector type
    Bacterial Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pT2/SVNeo
  • Insert Size (bp)
    4928
  • Mutation
    added the Rmut(80, 87, d101)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer M13(-20) Forward
  • 3′ sequencing primer M13 Reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT2/SVNeo was a gift from Perry Hackett (Addgene plasmid # 26553 ; http://n2t.net/addgene:26553 ; RRID:Addgene_26553)

  • For your References section:

    Structure-function analysis of the inverted terminal repeats of the sleeping beauty transposon. Cui Z, Geurts AM, Liu G, Kaufman CD, Hackett PB. J Mol Biol. 2002 May 17. 318(5):1221-35. 10.1016/S0022-2836(02)00237-1 PubMed 12083513
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