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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
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| Plasmid | 26552 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCMVbeta
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 3712
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSleeping Beauty
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Alt nameSB
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Alt nameSB11
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Insert Size (bp)1020
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MutationMethionine 243 to Glutamine Threonine 136 to Arginine Valine 253 to Histidine Alanine 255 to Arginine
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (destroyed during cloning)
- 3′ cloning site NotI (destroyed during cloning)
- 5′ sequencing primer M13 Forward
- 3′ sequencing primer M13 Reverse (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV/SB11 was a gift from Perry Hackett (Addgene plasmid # 26552 ; http://n2t.net/addgene:26552 ; RRID:Addgene_26552) -
For your References section:
Molecular reconstruction of Sleeping Beauty, a Tc1-like transposon from fish, and its transposition in human cells. Ivics Z, Hackett PB, Plasterk RH, Izsvak Z. Cell. 1997 Nov 14. 91(4):501-10. 10.1016/S0092-8674(00)80436-5 PubMed 9390559
Map uploaded by the depositor.
