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MXW-PGK-let-7_LPC-IRES-GFP
(Plasmid #26549)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26549 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MXW-PGK-let7-IRES-GFP
  • Backbone size w/o insert (bp) 7556
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    cel-pre-let-7_LPC
  • Alt name
    pre let-7 LPC
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    86
  • Mutation
    loop mutant C

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BstBI (not destroyed)
  • 5′ sequencing primer mPGK-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

BamHI and BstBI are endogenous sites that flank the hairpin region of wt cel-let-7.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MXW-PGK-let-7_LPC-IRES-GFP was a gift from Chang-Zheng Chen (Addgene plasmid # 26549 ; http://n2t.net/addgene:26549 ; RRID:Addgene_26549)

  • For your References section:

    The potential functions of primary microRNAs in target recognition and repression. Trujillo RD, Yue SB, Tang Y, O'Gorman WE, Chen CZ. EMBO J. 2010 Aug 31. ():. 10.1038/emboj.2010.208 PubMed 20808284
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