phRL-TKlk-lin-41_LCS-wt
(Plasmid
#26542)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 26542 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonephRL-TKlkF
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Backbone manufacturerChang-Zheng Chen Lab
- Backbone size w/o insert (bp) 4075
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namelin-41_LCS-wt
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Alt namelin-41 LCS wt
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SpeciesC. elegans (nematode)
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Insert Size (bp)108
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site SacI (not destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer EBV rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
phRL-TKlk-lin-41_LCS-wt was a gift from Chang-Zheng Chen (Addgene plasmid # 26542 ; http://n2t.net/addgene:26542 ; RRID:Addgene_26542) -
For your References section:
The potential functions of primary microRNAs in target recognition and repression. Trujillo RD, Yue SB, Tang Y, O'Gorman WE, Chen CZ. EMBO J. 2010 Aug 31. ():. 10.1038/emboj.2010.208 PubMed 20808284