Skip to main content
Addgene

MDH1-pre-let-7_PShairpin
(Plasmid #26537)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26537 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    MDH1-PGK-GFP
  • Backbone size w/o insert (bp) 6946
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    pre-let-7_PS hairpin
  • Alt name
    pre let-7 PS hairpin
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    85
  • Mutation
    5 nt mutated to make pre-let-7 stem pairing perfect

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer EGFP-C
  • 3′ sequencing primer H1
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MDH1-pre-let-7_PShairpin was a gift from Chang-Zheng Chen (Addgene plasmid # 26537 ; http://n2t.net/addgene:26537 ; RRID:Addgene_26537)

  • For your References section:

    The potential functions of primary microRNAs in target recognition and repression. Trujillo RD, Yue SB, Tang Y, O'Gorman WE, Chen CZ. EMBO J. 2010 Aug 31. ():. 10.1038/emboj.2010.208 PubMed 20808284
Related items: