pJC53.2
(Plasmid #26536)

• Purpose: (Empty Backbone) T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes
• Depositing Lab: Phillip Newmark
• Publication: Collins et al PLoS Biol. 2010 . 8(10):e1000509.

Backbone

• Vector backbone: pJC53.2
• Backbone size (bp): 3240
• Vector type: T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Kanamycin, 25 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DB3.1
• Growth instructions: Grow in invitrogen DB3.1 cells in presence of kanamycin and Chloramphenicol
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Insert Size (bp): 1600

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T3
• 3′ sequencing primer: SP6

Resource Information

• Articles Citing this Plasmid:
  • 11 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For cloning taq polymerase-amplified PCR products, digest vector with Eam1105I, purify, ligate with PCR product, and use to transform DH5-alpha cells (or strain of choice). dsRNA can be generated by in vitro transcription with T7 RNA polymerase and single stranded probes can be generated with SP6 or T3 RNA polymerases.