pmiReport PU.1 3' UTR
(Plasmid #26530)

• Depositing Lab: David Baltimore
• Publication: O'Connell et al J Exp Med. 2008 Mar 17. 205(3):585-94.

Backbone

• Vector backbone: pmiReport
• Backbone manufacturer: Ambion
• Backbone size w/o insert (bp): 6470
• Vector type: Mammalian Expression, Luciferase
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PU.1 3' UTR
• Alt name: Sfpi1
• Species: M. musculus (mouse)
• Insert Size (bp): 333
• Entrez Gene: Spi1 (a.k.a. Dis-1, Dis1, PU.1, Sfpi-1, Sfpi1, Spi-1, Tcfpu1, Tfpu.1)
• Tag / Fusion Protein:
  • Luciferase (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SpeI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: Luc-F
• 3′ sequencing primer: M13pUC-fwd

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The inserts put into this vector are downstream from luciferase. They create different 3' UTRs for luciferase.