MG-155
(Plasmid #26529)

• Depositing Lab: David Baltimore
• Publication: O'Connell et al J Exp Med. 2008 Mar 17. 205(3):585-94.

Backbone

• Vector backbone: pMIG-w
• Backbone manufacturer: Addgene plasmid 12282
• Backbone size w/o insert (bp): 6500
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: miR-155
• Alt name: BIC (part of Exon3)
• Species: H. sapiens (human)
• Insert Size (bp): 500
• Entrez Gene: MIR155 (a.k.a. MIRN155, miRNA155, mir-155)
• Tag / Fusion Protein:
  • eGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: pLXSN-5
• 3′ sequencing primer: MSCV-rev

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

MG155 was derived originally from pMIG-w. The GFP and IRES were removed using BglII and HindIII, and a new cassette with eGFP (about 700 bps) followed by a miR-155 sequence (about 500 bps with flanking Not1 and XhoI sites) was cloned in using BamHI (destroying the BglII site) and HindIII. This results in a 7 kb plasmid.