MG
(Plasmid #26528)

• Purpose: (Empty Backbone)
• Depositing Lab: David Baltimore
• Publication: O'Connell et al J Exp Med. 2008 Mar 17. 205(3):585-94.

Backbone

• Vector backbone: pMIG-w
• Backbone manufacturer: Addgene plasmid 12282
• Backbone size (bp): 6500
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: None
• Tag / Fusion Protein:
  • eGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: pLXSN-5
• 3′ sequencing primer: MSCV-rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

MG is derived from MG155 (see plasmid 26529). MG was created by removing miR-155 with Not1 and Xho1, filling in the ends, and ligating the plasmid back together.