MGP M155
(Plasmid #26527)

• Depositing Lab: David Baltimore
• Publication: O'Connell et al Proc Natl Acad Sci U S A. 2009 Apr 28. 106(17):7113-8.

Backbone

• Vector backbone: pMSCV puro
• Backbone size w/o insert (bp): 6300
• Vector type: Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: miR-155
• Alt name: BIC (miRNA containing region of Exon2)
• Species: M. musculus (mouse)
• Entrez Gene: Mir155 (a.k.a. Mirn155, mir-155, mmu-mir-155)
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: pLXSN-5
• 3′ sequencing primer: MSCV-rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pMGP is a modified MSCV vector whereby GFP was placed downstream from the 5′ LTR, and the miR-155 expression cassette was cloned downstream from the GFP stop codon.