pJC100.1-ppl-1
(Plasmid
#26519)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 26519 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepJC53.2
- Backbone size w/o insert (bp) 3240
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Vector typeRNAi
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameppl-1
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SpeciesS. mediterranea (planarian)
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Insert Size (bp)334
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GenBank IDBK007007
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Eam1105I (destroyed during cloning)
- 3′ cloning site Eam1105I (destroyed during cloning)
- 5′ sequencing primer Sp6
- 3′ sequencing primer T3 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJC100.1-ppl-1 was a gift from Phillip Newmark (Addgene plasmid # 26519 ; http://n2t.net/addgene:26519 ; RRID:Addgene_26519) -
For your References section:
Genome-wide analyses reveal a role for peptide hormones in planarian germline development. Collins JJ, Hou X, Romanova EV, Lambrus BG, Miller CM, Saberi A, Sweedler JV, Newmark PA. PLoS Biol. 2010 . 8(10):e1000509. 10.1371/journal.pbio.1000509 PubMed 20967238