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pJC54.2-npp-22
(Plasmid #26493)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26493 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJC53.2
  • Backbone size w/o insert (bp) 3240
  • Vector type
    T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    npp-22
  • Species
    S. mediterranea (planarian)
  • Insert Size (bp)
    472
  • GenBank ID
    BK007038

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Eam1105I (destroyed during cloning)
  • 3′ cloning site Eam1105I (destroyed during cloning)
  • 5′ sequencing primer T3
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    EST PL05004B1G05 in pBluescript

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

dsRNA can be generated by in vitro transcription with T7 RNA polymerase and single stranded probes can be generated with SP6 or T3 RNA polymerases.

The insert has minor sequence discrepancies with NCBI; these do not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJC54.2-npp-22 was a gift from Phillip Newmark (Addgene plasmid # 26493 ; http://n2t.net/addgene:26493 ; RRID:Addgene_26493)

  • For your References section:

    Genome-wide analyses reveal a role for peptide hormones in planarian germline development. Collins JJ, Hou X, Romanova EV, Lambrus BG, Miller CM, Saberi A, Sweedler JV, Newmark PA. PLoS Biol. 2010 . 8(10):e1000509. 10.1371/journal.pbio.1000509 PubMed 20967238
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