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MXW-PGK-let-7_wt-IRES-GFP
(Plasmid #26491)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26491 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MXW-PGK-IRES-GFP
  • Backbone manufacturer
    Chang-Zheng Chen Lab
  • Backbone size w/o insert (bp) 7060
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    cel-let-7 (~250nt flanking each side)
  • Alt name
    cel let-7
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    582

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer mPGK-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Ordinarily, we express miRNAs from the LTR, which is pol III. However, cel-let-7 won't express from that promoter (possibly due to long poly-T tracts). This is a pol II expression system.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MXW-PGK-let-7_wt-IRES-GFP was a gift from Chang-Zheng Chen (Addgene plasmid # 26491 ; http://n2t.net/addgene:26491 ; RRID:Addgene_26491)

  • For your References section:

    The potential functions of primary microRNAs in target recognition and repression. Trujillo RD, Yue SB, Tang Y, O'Gorman WE, Chen CZ. EMBO J. 2010 Aug 31. ():. 10.1038/emboj.2010.208 PubMed 20808284
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