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Addgene

repressilator (pZS1-lTlrLLtCL)
(Plasmid #26489)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26489 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pZS1
  • Backbone size w/o insert (bp) 3552
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    MG1655-Z1
  • Growth instructions
    plasmid is in MG1655Z1 which is spectR
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    LacI, tetR
  • Species
    e.coli
  • Insert Size (bp)
    6664

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site BamH1 (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    repressilator (pZS1-lTlrLLtCL) was a gift from Michael Elowitz (Addgene plasmid # 26489 ; http://n2t.net/addgene:26489 ; RRID:Addgene_26489)

  • For your References section:

    A synthetic oscillatory network of transcriptional regulators. Elowitz MB, Leibler S. Nature. 2000 Jan 20. 403(6767):335-8. 10.1038/35002125 PubMed 10659856
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