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phRL-TKlk-lin-41_LCS-sm
(Plasmid #26487)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26487 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    phRL-TKlkF
  • Backbone manufacturer
    Chang-Zheng Chen Lab
  • Backbone size w/o insert (bp) 4075
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    lin-41_LCS-sm
  • Alt name
    lin-41 LCS-sm
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    108
  • Mutation
    Seed regions of both target sites are mutated.

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site SacI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer EBV rev
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Negative control for luciferase assays (to measure seed-dependent regulation). Renilla luciferase.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    phRL-TKlk-lin-41_LCS-sm was a gift from Chang-Zheng Chen (Addgene plasmid # 26487 ; http://n2t.net/addgene:26487 ; RRID:Addgene_26487)

  • For your References section:

    The potential functions of primary microRNAs in target recognition and repression. Trujillo RD, Yue SB, Tang Y, O'Gorman WE, Chen CZ. EMBO J. 2010 Aug 31. ():. 10.1038/emboj.2010.208 PubMed 20808284
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