pCBASceI
(Plasmid #26477)

• Purpose: I-SceI endonuclease expression vector with mammalian promoter to introduce a DSB at a genomic I-SceI site
• Depositing Lab: Maria Jasin
• Publication: Richardson et al Genes Dev. 1998 Dec 15. 12(24):3831-42.

Backbone

• Vector backbone: pCAGGS
• Backbone size w/o insert (bp): 3073
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pCBASceI
• Alt name: CBAS
• Insert Size (bp): 2583
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (destroyed during cloning)
• 3′ cloning site: XhoI (destroyed during cloning)
• 5′ sequencing primer: n/a

Resource Information

• Articles Citing this Plasmid:
  • 159 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry