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PurposeDR-GFP substrate (Addgene 26475) flanked by mouse hprt targeting sequences
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 26476 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepgkPURO
- Backbone size w/o insert (bp) 1480
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Vector typeMammalian Expression, Mouse Targeting
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepHPRT-DRGFP
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Insert Size (bp)10987
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHPRT-DRGFP was a gift from Maria Jasin (Addgene plasmid # 26476 ; http://n2t.net/addgene:26476 ; RRID:Addgene_26476) -
For your References section:
Ku DNA end-binding protein modulates homologous repair of double-strand breaks in mammalian cells. Pierce AJ, Hu P, Han M, Ellis N, Jasin M. Genes Dev. 2001 Dec 15. 15(24):3237-42. 10.1101/gad.946401 PubMed 11751629
Map uploaded by the depositor.
