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PurposeIn vivo homologous recombination substrate composed of two differentially mutated GFP genes oriented as direct repeats and separated by a drug selection marker
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 26475 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepgkPURO
- Backbone size w/o insert (bp) 4258
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDRGFP
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Insert Size (bp)8646
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer None (Common Sequencing Primers)
Resource Information
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Addgene Notes
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDRGFP was a gift from Maria Jasin (Addgene plasmid # 26475 ; http://n2t.net/addgene:26475 ; RRID:Addgene_26475) -
For your References section:
XRCC3 promotes homology-directed repair of DNA damage in mammalian cells. Pierce AJ, Johnson RD, Thompson LH, Jasin M. Genes Dev. 1999 Oct 15. 13(20):2633-8. 10.1101/gad.13.20.2633 PubMed 10541549
Map uploaded by the depositor.
