pPDS1-mEGFP-IRES-puro2b
(Plasmid #26464)

• Depositing Lab: Daniel Gerlich
• Publication: Held et al Nat Methods. 2010 Sep . 7(9):747-54.

Backbone

• Vector backbone: pIRES-puro2b
• Backbone size w/o insert (bp): 5844
• Vector type: Mammalian Expression, Bacterial Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pPDS1
• Alt name: PTTG1
• Alt name: Securin
• Species: H. sapiens (human)
• Insert Size (bp): 614
• Entrez Gene: PTTG1 (a.k.a. EAP1, ECRAR, HPTTG, PTTG, TUTR1)
• Tag / Fusion Protein:
  • mEGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nde1 (unknown if destroyed)
• 3′ cloning site: BamH1 (unknown if destroyed)
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry