pIBB-mEGFP-IRES-puro2b
(Plasmid #26462)

• Depositing Lab: Daniel Gerlich
• Publication: Held et al Nat Methods. 2010 Sep . 7(9):747-54.

Backbone

• Vector backbone: pIRES-puro2b
• Backbone size w/o insert (bp): 5829
• Vector type: Mammalian Expression, Bacterial Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pIBB
• Species: H. sapiens (human)
• Insert Size (bp): 196
• Tag / Fusion Protein:
  • mEGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (unknown if destroyed)
• 3′ cloning site: Not1 (unknown if destroyed)
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There are some discrepancies between the author's sequence and Addgene's quality control sequence, but the depositing lab says that the plasmids should function normally.