pEGFP-PCNA-IRES-puro2b
(Plasmid #26461)

• Depositing Lab: Daniel Gerlich
• Publication: Held et al Nat Methods. 2010 Sep . 7(9):747-54.

Backbone

• Vector backbone: pIRES-puro2b
• Backbone size w/o insert (bp): 6037
• Vector type: Mammalian Expression, Bacterial Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PCNA
• Species: H. sapiens (human)
• Insert Size (bp): 758
• GenBank ID: NP_002583.1
• Entrez Gene: PCNA (a.k.a. ATLD2)
• Tag / Fusion Protein:
  • pEGFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nde1 (unknown if destroyed)
• 3′ cloning site: Xba1 (unknown if destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note that there are a few minor discrepancies between Addgene's quality control sequence and the author's sequence, but these should not affect function.